Optimization of phenol and flavonoid extraction compound of Adiantum capillus-veneris L. using ultrasound assisted extraction

Background: Antioxidants are molecules which able to control free radicals and prevent the oxidants of other molecules considering undesirable effects of synthetic anti oxidant. In recent years using of natural anti oxidant gets the great importance. The secondary metabolisms of plants are rich sources of phenol and flavonoids considered as the most important natural antioxidant.  Objective: In this research, the optimization process of phenolic and flavonoids from Adiantum capillus-veneris L. carried out by ultrasound-assisted extraction (UAE) and central composite design (CCD). Methods: Optimization process were performed with four indepent variables including, plant powder (0.1-0.9 g), volume of solvent (5-17 ml), sonication time (5–25 min) and temperature of ultrassonic bath (20-60 ºC) on five levels including 26 test with 2 repetition on central point. The total phenolic content and total flavonoid content, respectively, were accounted by using foline-ciocalteu and aluminum chloride colorimetric method. Results: At optimum conditions values of variables set at 0.7 g of plant powder, 16 ml of extraction solvent, 20 min for ultrasound waves and 40 ºC for ultrasonic bath. Conclusion: The results show that the quadratic mathematical model has acted with high accuracy to predict the optimal values of the studied parameters. Under these conditions, the extraction efficiencies for total phenol and flavonoid compounds were 9.46 mg/g and 5.22 mg/g, respectively.